Gene Details


gene name: narH


locus tag k12ECK1219 Crenarchaeota Euryarchaeota Nanoarcheota Alphaproteobacteria Betaproteobacteria Gammaproteobacteria Deltaproteobacteria Epsilonproteobacteria Cyanobacteria Actinobacteridae Firmicutes Spirochaetes others Protist Microsporidia Fungi Metazoa Plantae
information on phylogenetic profile
locus tag mg1655b1225
locus tag w3110JW1216
gene name k12narH
locus namenarH
synonyms of locus namechlC

scop id54862
superfamily4Fe-4S ferredoxins
pfam idPF00037
pfam domain4Fe-4S binding domain
tigrfam idTIGR01660
namenarH
functionnitrate reductase, beta subunit

swissprot nameNARH_ECOLI
descriptionRespiratory nitrate reductase 1 beta chain (EC 1.7.99.4) (Nitrate|reductase A subunit beta) (Quinol-nitrate oxidoreductase subunit|beta).
seq length512
fastaseq

go idGO:0009274; GO:0019866; GO:0009054; GO:0009061; GO:0009061
go termcell wall (sensu Bacteria); inner membrane; electron acceptor activity; anaerobic respiration; anaerobic resp

gene product descriptionnitrate reductase 1, beta (Fe-S) subunit
comment gene product description
evidenceE
context
gene product descCarrier
cell locationCytoplasmic
featuresCDS

functional category codeC
functional categoryEnergy production and conversion

reference #1The bacterial nitrate reductases. Solubilization, purification and properties of the enzyme A of Escherichia coli K 12.

Forget P. (Eur J Biochem. 1974 Mar 1; 42(2):325-32)
reference #2Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the alpha and beta subunits in iron binding and electron transfer.

Blasco F., Iobbi C., Giordano G., Chippaux M., Bonnefoy V. (Mol Gen Genet. 1989 Aug; 218(2):249-56)
reference #3The narJ gene product is required for biogenesis of respiratory nitrate reductase in Escherichia coli.

Dubourdieu M., DeMoss J. (J Bacteriol. 1992 Feb; 174(3):867-72)
reference #4EPR and redox characterization of iron-sulfur centers in nitrate reductases A and Z from Escherichia coli. Evidence for a high-potential and a low-potential class and their relevance in the electron-transfer mechanism.

Guigliarelli B., Asso M., More C., Augier V., Blasco F., Pommier J., Giordano G., Bertrand P. (Eur J Biochem. 1992 Jul 1; 207(1):61-8)
reference #5narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes.

Sodergren E., DeMoss J. (J Bacteriol. 1988 Apr; 170(4):1721-9)
reference #6Roles of the narJ and narI gene products in the expression of nitrate reductase in Escherichia coli.

Sodergren E., Hsu P., DeMoss J. (J Biol Chem. 1988 Nov 5; 263(31):16156-62)
reference #7Site-directed mutagenesis of conserved cysteine residues within the beta subunit of Escherichia coli nitrate reductase. Physiological, biochemical, and EPR characterization of the mutated enzymes.

Augier V., Guigliarelli B., Asso M., Bertrand P., Frixon C., Giordano G., Chippaux M., Blasco F. (Biochemistry. 1993 Mar 2; 32(8):2013-23)
reference #8Removal of the high-potential [4Fe-4S] center of the beta-subunit from Escherichia coli nitrate reductase. Physiological, biochemical, and EPR characterization of site-directed mutated enzymes.

Augier V., Asso M., Guigliarelli B., More C., Bertrand P., Santini C., Blasco F., Chippaux M., Giordano G. (Biochemistry. 1993 May 18; 32(19):5099-108)
reference #9Complete coordination of the four Fe-S centers of the beta subunit from Escherichia coli nitrate reductase. Physiological, biochemical, and EPR characterization of site-directed mutants lacking the highest or lowest potential [4Fe-4S] clusters.

Guigliarelli B., Magalon A., Asso M., Bertrand P., Frixon C., Giordano G., Blasco F. (Biochemistry. 1996 Apr 16; 35(15):4828-36)
reference #10The molybdenum cofactor of Escherichia coli nitrate reductase A (NarGHI). Effect of a mobAB mutation and interactions with [Fe-S] clusters.

Rothery R., Magalon A., Giordano G., Guigliarelli B., Blasco F., Weiner J. (J Biol Chem. 1998 Mar 27; 273(13):7462-9)

phylogenetic profile
 
514 531 83 87 99 87 51 56 70 1 1 1 1 57 1 1 1 1 1 1
571 586 592 1 562 1 562 562 558 565 1 568 95 1 1 1 1 1 50 791
794 1 89 1 1 1 1 60 1 106 106 1 761 763 773 1 1 1 763 1
1 1 1 1 1 1 1 55 77 82 93 1 52 1 1 99 563 630 1 1
636 637 1 59 1 1 1 610 1 1 1 1 1 1 1 1 1 1 1 1
1 1 1 1 638 634 632 634 632 1 1 1 1 1 1 1 1 1 1 57
1 1 1 1 1 1 1 942 1072 1070 1070 84 1 1 79 67 97 65 815 72
1 1006 1004 1006 79 1072 1072 53 96 99 1 1 1 1 1 95 95 95 89 1
1 1 1 1 1 1 1 73 1 1 1 64 1 58 87 1 1 1 1 1
1 1 1 1 1 1 1 1 74 1 1 1 1 1 1 1 1 1 1