Gene Details


gene name: ogt


locus tag k12ECK1331 Crenarchaeota Euryarchaeota Nanoarcheota Alphaproteobacteria Betaproteobacteria Gammaproteobacteria Deltaproteobacteria Epsilonproteobacteria Cyanobacteria Actinobacteridae Firmicutes Spirochaetes others Protist Microsporidia Fungi Metazoa Plantae
information on phylogenetic profile
locus tag mg1655b1335
locus tag w3110JW1329
gene name k12ogt
locus nameogt
synonyms of locus name

scop id46767
superfamilyMethylated DNA-protein cysteine methyltransferase, C-terminal domain
pfam idPF02870; PF01035
pfam domain6-O-methylguanine DNA methyltransferase, ribonuclease-like domain; 6-O-methylguanine DNA methyltransferase, DNA binding domain
tigrfam idTIGR00589
nameogt
functionmethylated-DNA--protein-cysteine methyl

swissprot nameOGT_ECOLI
descriptionMethylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-|methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-|alkyltransferase).
seq length171
fastaseq

go idGO:0005737; GO:0006306
go termcytoplasm; DNA methylation

gene product descriptionO-6-alkylguanine-DNA:cysteine-protein methyltransferase
comment gene product description
evidenceE
context
gene product descEnzyme
cell locationCytoplasmic
featuresCDS

functional category codeL
functional categoryReplication, recombination and repair

reference #1Characterisation and nucleotide sequence of ogt, the O6-alkylguanine-DNA-alkyltransferase gene of E. coli.

Potter P., Wilkinson M., Fitton J., Carr F., Brennand J., Cooper D., Margison G. (Nucleic Acids Res. 1987 Nov 25; 15(22):9177-93)
reference #2Increased spontaneous mutation and alkylation sensitivity of Escherichia coli strains lacking the ogt O6-methylguanine DNA repair methyltransferase.

Rebeck G., Samson L. (J Bacteriol. 1991 Mar; 173(6):2068-76)
reference #3DNA alkylation repair limits spontaneous base substitution mutations in Escherichia coli.

Mackay W., Han S., Samson L. (J Bacteriol. 1994 Jun; 176(11):3224-30)
reference #4Influence of DNA repair by (A)BC excinuclease and Ogt alkyltransferase on the distribution of mutations induced by n-propyl-N-nitrosourea in Escherichia coli.

Ferrezuelo F., Prieto-Alamo M., Jurado J., Pueyo C. (Environ Mol Mutagen. 1998; 31(1):82-91)
reference #5Site directed mutagenesis of two cysteine residues in the E. coli ogt O6-alkylguanine DNA alkyltransferase protein.

Harris L., Potter P., Margison G. (Biochem Biophys Res Commun. 1992 Aug 31; 187(1):425-31)

phylogenetic profile
 
1 1 59 1 1 1 58 1 52 50 97 97 1 1 1 1 1 1 1 72
70 60 62 82 96 86 96 96 65 71 99 93 59 1 1 105 66 72 143 89
89 134 75 69 76 1 1 105 1 132 129 132 72 72 94 81 81 108 97 140
87 1 1 1 1 1 1 84 81 64 75 65 83 88 86 1 103 82 103 95
80 82 82 92 95 79 83 75 72 94 91 90 90 91 1 1 1 1 1 70
1 75 82 1 71 70 70 70 70 86 89 92 89 89 1 1 1 1 1 79
1 96 1 1 1 1 65 187 318 322 322 85 89 88 87 72 86 88 100 92
72 293 293 293 83 323 323 97 94 94 1 90 89 76 79 188 188 188 1 81
84 1 1 1 1 1 1 72 1 57 73 63 90 61 62 1 56 77 63 56
1 62 52 73 1 66 1 1 89 63 61 58 69 65 1 1 1 1 1