Gene Details


gene name: ogt


locus tag k12ECK1331 Crenarchaeota Euryarchaeota Nanoarcheota Alphaproteobacteria Betaproteobacteria Gammaproteobacteria Deltaproteobacteria Epsilonproteobacteria Cyanobacteria Actinobacteridae Firmicutes Spirochaetes others Protist Microsporidia Fungi Metazoa Plantae
information on phylogenetic profile
locus tag mg1655b1335
locus tag w3110JW1329
gene name k12ogt
locus nameogt
synonyms of locus name

scop id46767
superfamilyMethylated DNA-protein cysteine methyltransferase, C-terminal domain
pfam idPF01035; PF02870
pfam domain6-O-methylguanine DNA methyltransferase, DNA binding domain; 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain
tigrfam idTIGR00589
nameogt
functionmethylated-DNA--protein-cysteine methyl

swissprot nameOGT_ECOLI
descriptionMethylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-|methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-|alkyltransferase).
seq length171
fastaseq

go idGO:0005737; GO:0006306
go termcytoplasm; DNA methylation

gene product descriptionO-6-alkylguanine-DNA:cysteine-protein methyltransferase
comment gene product description
evidenceE
context
gene product descEnzyme
cell locationCytoplasmic
featuresCDS

functional category codeL
functional categoryReplication, recombination and repair

reference #1Site directed mutagenesis of two cysteine residues in the E. coli ogt O6-alkylguanine DNA alkyltransferase protein.

Harris L., Potter P., Margison G. (Biochem Biophys Res Commun. 1992 Aug 31; 187(1):425-31)
reference #2Characterisation and nucleotide sequence of ogt, the O6-alkylguanine-DNA-alkyltransferase gene of E. coli.

Potter P., Wilkinson M., Fitton J., Carr F., Brennand J., Cooper D., Margison G. (Nucleic Acids Res. 1987 Nov 25; 15(22):9177-93)
reference #3Increased spontaneous mutation and alkylation sensitivity of Escherichia coli strains lacking the ogt O6-methylguanine DNA repair methyltransferase.

Rebeck G., Samson L. (J Bacteriol. 1991 Mar; 173(6):2068-76)
reference #4DNA alkylation repair limits spontaneous base substitution mutations in Escherichia coli.

Mackay W., Han S., Samson L. (J Bacteriol. 1994 Jun; 176(11):3224-30)
reference #5Influence of DNA repair by (A)BC excinuclease and Ogt alkyltransferase on the distribution of mutations induced by n-propyl-N-nitrosourea in Escherichia coli.

Ferrezuelo F., Prieto-Alamo M., Jurado J., Pueyo C. (Environ Mol Mutagen. 1998; 31(1):82-91)

phylogenetic profile
 
1 1 1 59 58 1 1 1 1 1 1 1 97 97 50 52 1 1 1 96
72 59 82 62 93 99 96 60 96 70 71 1 1 65 86 66 69 1 105 143
89 89 75 134 105 1 1 76 72 132 132 129 72 72 94 81 81 108 97 1
1 1 140 1 87 1 1 84 64 81 75 88 86 65 1 83 72 70 71 82
80 75 1 83 94 91 90 90 91 79 95 92 82 70 103 82 103 86 95 1
1 1 1 1 1 1 1 1 70 1 75 89 89 1 1 92 89 82 70 79
70 188 187 318 322 322 85 89 88 65 87 72 188 188 86 88 1 100 92 72
1 1 1 293 293 293 83 323 323 97 94 94 1 96 90 89 76 79 63 73
1 1 1 1 1 1 72 1 61 62 57 1 81 84 90 63 56 1 56 77
1 66 73 1 62 52 1 1 61 63 89 58 65 69 1 1 1 1 1