Gene Details


gene name: nfo


locus tag k12ECK2152 Crenarchaeota Euryarchaeota Nanoarcheota Alphaproteobacteria Betaproteobacteria Gammaproteobacteria Deltaproteobacteria Epsilonproteobacteria Cyanobacteria Actinobacteridae Firmicutes Spirochaetes others Protist Microsporidia Fungi Metazoa Plantae
information on phylogenetic profile
locus tag mg1655b2159
locus tag w3110JW2146
gene name k12nfo
locus namenfo
synonyms of locus name

scop id51658
superfamilyXylose isomerase-like
pfam idPF01261
pfam domainXylose isomerase-like TIM barrel
tigrfam idTIGR00587
namenfo
functionapurinic endonuclease (APN1)

swissprot nameEND4_ECOLI
descriptionEndonuclease 4 (EC 3.1.21.2) (Endonuclease IV) (Endodeoxyribonuclease|IV).
seq length285
fastaseq

go idGO:0009314; GO:0006308
go termresponse to radiation; DNA catabolism

gene product descriptionendonuclease IV with intrinsic 3'-5' exonuclease activity
comment gene product description
evidenceE
context
gene product descEnzyme
cell locationPeriplasmic
featuresCDS

functional category codeL
functional categoryReplication, recombination and repair

reference #1Characterization of an endonuclease IV 3'-5' exonuclease activity.

Kerins S., Collins R., McCarthy T. (J Biol Chem. 2003 Jan 31; 278(5):3048-54)
reference #2Homogeneous Escherichia coli endonuclease IV. Characterization of an enzyme that recognizes oxidative damage in DNA.

Levin J., Johnson A., Demple B. (J Biol Chem. 1988 Jun 15; 263(17):8066-71)
reference #3Recognition of oxidized abasic sites by repair endonucleases.

Häring M., Rüdiger H., Demple B., Boiteux S., Epe B. (Nucleic Acids Res. 1994 Jun 11; 22(11):2010-5)
reference #4Interactions of Escherichia coli endonuclease IV and exonuclease III with abasic sites in DNA.

Takeuchi M., Lillis R., Demple B., Takeshita M. (J Biol Chem. 1994 Aug 26; 269(34):21907-14)
reference #5Endonuclease III and endonuclease IV protect Escherichia coli from the lethal and mutagenic effects of near-UV irradiation.

Serafini D., Schellhorn H. (Can J Microbiol. 1999 Jul; 45(7):632-7)
reference #6Structure of the DNA repair enzyme endonuclease IV and its DNA complex: double-nucleotide flipping at abasic sites and three-metal-ion catalysis.

Hosfield D., Guan Y., Haas B., Cunningham R., Tainer J. (Cell. 1999 Aug 6; 98(3):397-408)
reference #7Metalloenzymes in DNA repair. Escherichia coli endonuclease IV and Saccharomyces cerevisiae Apn1.

Levin J., Shapiro R., Demple B. (J Biol Chem. 1991 Dec 5; 266(34):22893-8)
reference #8Nucleotide sequence of the nfo gene of Escherichia coli K-12.

Saporito S., Cunningham R. (J Bacteriol. 1988 Nov; 170(11):5141-5)

phylogenetic profile
 
50 1 1 1 1 132 1 1 1 1 1 1 98 1 1 1 154 149 1 121
1 1 1 1 70 71 70 70 65 53 130 121 120 1 1 1 1 1 1 1
1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
1 1 1 1 1 1 1 1 348 145 190 1 1 1 1 313 116 116 117 124
134 131 1 52 1 128 1 120 1 127 124 125 124 125 135 118 128 115 115 140
125 105 126 112 128 128 128 128 128 1 1 1 1 1 1 1 1 1 1 1
118 1 1 289 331 341 1 433 525 528 529 1 1 1 1 1 1 403 1 1
1 476 477 476 1 525 525 355 347 337 1 1 1 1 1 426 426 426 141 336
329 192 192 192 190 192 200 312 1 1 216 1 1 154 113 1 1 1 1 1
209 220 205 210 213 205 185 215 1 237 239 1 1 1 215 1 1 237 232