Gene Details


gene name: ispH


locus tag k12ECK0030 Crenarchaeota Euryarchaeota Nanoarcheota Alphaproteobacteria Betaproteobacteria Gammaproteobacteria Deltaproteobacteria Epsilonproteobacteria Cyanobacteria Actinobacteridae Firmicutes Spirochaetes others Protist Microsporidia Fungi Metazoa Plantae
information on phylogenetic profile
locus tag mg1655b0029
locus tag w3110JW0027
gene name k12ispH
locus nameispH
synonyms of locus namelytB, yaaE

SCOPno information
pfam idPF02401
pfam domainLytB protein
tigrfam idTIGR00216
nameispH_lytB
function4-hydroxy-3-methylbut-2-enyl dip

swissprot nameISPH_ECOLI
description4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2).
seq length316
fastaseq

go idGO:0005737; GO:0042493
go termcytoplasm; response to drug

gene product description1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase, 4Fe-4S protein
comment gene product description
evidenceE
context
gene product descCarrier
cell locationCytoplasmic
featuresCDS

functional category codeM; I
functional categoryCell wall/membrane/envelope biogenesis ; Lipid transport and metabolism

reference #1Biosynthesis of terpenes: studies on 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase.

Adam P., Hecht S., Eisenreich W., Kaiser J., Grawert T., Arigoni D., Bacher A., Rohdich F. (Proc Natl Acad Sci U S A. 2002 Sep 17; 99(19):12108-13)
reference #2Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein.

Wolff M., Seemann M., Tse Sum Bui B., Frapart Y., Tritsch D., Garcia Estrabot A., Rodríguez-Concepción M., Boronat A., Marquet A., Rohmer M. (FEBS Lett. 2003 Apr 24; 541(1-3):115-20)
reference #3LytB, a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli.

Altincicek B., Kollas A., Eberl M., Wiesner J., Sanderbrand S., Hintz M., Beck E., Jomaa H. (FEBS Lett. 2001 Jun 15; 499(1-2):37-40)
reference #4The lytB gene of Escherichia coli is essential and specifies a product needed for isoprenoid biosynthesis.

McAteer S., Coulson A., McLennan N., Masters M. (J Bacteriol. 2001 Dec; 183(24):7403-7)
reference #5Studies on the nonmevalonate terpene biosynthetic pathway: metabolic role of IspH (LytB) protein.

Rohdich F., Hecht S., Gärtner K., Adam P., Krieger C., Amslinger S., Arigoni D., Bacher A., Eisenreich W. (Proc Natl Acad Sci U S A. 2002 Feb 5; 99(3):1158-63)
reference #6Identification of the Escherichia coli lytB gene, which is involved in penicillin tolerance and control of the stringent response.

Gustafson C., Kaul S., Ishiguro E. (J Bacteriol. 1993 Feb; 175(4):1203-5)
reference #7Occurrence of homologs of the Escherichia coli lytB gene in gram-negative bacterial species.

Potter S., Yang X., Boulanger M., Ishiguro E. (J Bacteriol. 1998 Apr; 180(7):1959-61)

phylogenetic profile
 
1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 249
277 277 278 289 291 277 291 291 296 277 302 305 167 269 270 266 266 261 317 270
270 293 263 309 1 1 1 268 277 347 347 347 337 337 343 340 340 368 337 70
81 76 87 82 79 80 77 1 277 103 134 114 106 119 119 122 161 1 161 164
161 166 135 122 112 1 1 1 1 1 153 152 151 152 1 1 1 1 1 125
1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 135
1 386 1 158 333 337 1 487 582 582 582 409 1 1 433 395 429 476 389 386
390 550 550 550 391 573 580 391 389 387 325 361 358 359 359 493 493 491 149 102
102 247 247 247 241 244 241 84 212 123 269 274 84 108 211 1 236 94 80 236
1 1 1 1 1 1 1 1 142 1 1 1 1 1 1 1 66 1 210