Gene Details


gene name: dnaJ


locus tag k12ECK0015 Crenarchaeota Euryarchaeota Nanoarcheota Alphaproteobacteria Betaproteobacteria Gammaproteobacteria Deltaproteobacteria Epsilonproteobacteria Cyanobacteria Actinobacteridae Firmicutes Spirochaetes others Protist Microsporidia Fungi Metazoa Plantae
information on phylogenetic profile
locus tag mg1655b0015
locus tag w3110JW0014
gene name k12dnaJ
locus namednaJ
synonyms of locus namegroP, grpC

SCOPno information
pfam idPF00684; PF00226; PF01556
pfam domainDnaJ central domain (4 repeats); DnaJ domain; DnaJ C terminal region
TIGRFAMno information

swissprot nameDNAJ_ECOLI
descriptionChaperone protein dnaJ (Heat shock protein J) (HSP40).
seq length375
fastaseq

go idGO:0005737; GO:0006457
go termcytoplasm; protein folding

gene product descriptionchaperone Hsp40, co-chaperone with DnaK
comment gene product description
evidenceE
context
gene product descFactor
cell locationCytoplasmic
featuresCDS

functional category codeO
functional categoryPosttranslational modification, protein turnover, chaperones

reference #1Successive action of DnaK, DnaJ and GroEL along the pathway of chaperone-mediated protein folding.

Langer T., Lu C., Echols H., Flanagan J., Hayer M., Hartl F. (Nature. 1992 Apr 23; 356(6371):683-9)
reference #2Physical interaction between heat shock proteins DnaK, DnaJ, and GrpE and the bacterial heat shock transcription factor sigma 32.

Gamer J., Bujard H., Bukau B. (Cell. 1992 May 29; 69(5):833-42)
reference #3DnaK, DnaJ and GrpE form a cellular chaperone machinery capable of repairing heat-induced protein damage.

Schröder H., Langer T., Hartl F., Bukau B. (EMBO J. 1993 Nov; 12(11):4137-44)
reference #4Levels of DnaK and DnaJ provide tight control of heat shock gene expression and protein repair in Escherichia coli.

Tomoyasu T., Ogura T., Tatsuta T., Bukau B. (Mol Microbiol. 1998 Nov; 30(3):567-81)
reference #5The influence of C-terminal extension on the structure of the "J-domain" in E. coli DnaJ.

Huang K., Flanagan J., Prestegard J. (Protein Sci. 1999 Jan; 8(1):203-14)
reference #6Solution structure of the cysteine-rich domain of the Escherichia coli chaperone protein DnaJ.

Martinez-Yamout M., Legge G., Zhang O., Wright P., Dyson H. (J Mol Biol. 2000 Jul 21; 300(4):805-18)
reference #7Escherichia coli DnaJ and GrpE heat shock proteins jointly stimulate ATPase activity of DnaK.

Liberek K., Marszalek J., Ang D., Georgopoulos C., Zylicz M. (Proc Natl Acad Sci U S A. 1991 Apr 1; 88(7):2874-8)
reference #8Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product.

Ohki M., Tamura F., Nishimura S., Uchida H. (J Biol Chem. 1986 Feb 5; 261(4):1778-81)
reference #9The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein.

Bardwell J., Tilly K., Craig E., King J., Zylicz M., Georgopoulos C. (J Biol Chem. 1986 Feb 5; 261(4):1782-5)
reference #10NMR structure of the J-domain and the Gly/Phe-rich region of the Escherichia coli DnaJ chaperone.

Pellecchia M., Szyperski T., Wall D., Georgopoulos C., Wüthrich K. (J Mol Biol. 1996 Jul 12; 260(2):236-50)

phylogenetic profile
 
1 1 1 1 1 160 254 1 1 1 240 239 182 1 1 1 176 172 1 175
191 196 186 182 191 186 191 191 192 214 209 210 263 173 174 310 294 286 280 307
307 281 299 288 252 255 254 306 246 335 333 326 347 348 356 332 332 370 337 206
199 198 202 201 205 202 224 255 288 247 314 199 194 193 186 216 268 264 267 259
266 256 259 262 261 245 216 226 228 254 255 254 255 254 125 125 73 127 176 117
124 150 268 117 241 241 241 241 241 237 238 248 244 246 240 240 241 241 241 274
161 331 437 420 422 440 350 509 570 570 570 375 366 366 387 371 381 485 395 386
378 546 546 546 422 566 571 430 440 433 420 355 353 350 350 505 505 505 200 168
168 234 234 234 239 214 196 169 210 260 244 243 198 182 193 202 259 239 60 259
125 131 128 128 140 115 166 127 117 180 167 132 191 188 79 210 181 107 159