Gene Details


gene name: carB


locus tag k12ECK0034 Crenarchaeota Euryarchaeota Nanoarcheota Alphaproteobacteria Betaproteobacteria Gammaproteobacteria Deltaproteobacteria Epsilonproteobacteria Cyanobacteria Actinobacteridae Firmicutes Spirochaetes others Protist Microsporidia Fungi Metazoa Plantae
information on phylogenetic profile
locus tag mg1655b0033
locus tag w3110JW0031
gene name k12carB
locus namecarB
synonyms of locus namearg+ura, cap, pyrA

scop id56059; 52440; 52335; 48108
superfamilyGlutathione synthetase ATP-binding domain-like; Biotin carboxylase N-terminal domain-like; Methylglyoxal synthase-like; Carbamoyl phosphate synthetase, large subunit connection domain
pfam idPF02142; PF02787; PF02786; PF00289
pfam domainMGS-like domain; Carbamoyl-phosphate synthetase large chain; Carbamoyl-phosphate synthase L chain,; Carbamoyl-phosphate synthase L chain,
tigrfam idTIGR01369
nameCPSaseII_lrg
functioncarbamoyl-phosphate syntha

swissprot nameCARB_ECOLI
descriptionCarbamoyl-phosphate synthase large chain (EC 6.3.5.5) (Carbamoyl-|phosphate synthetase ammonia chain).
seq length1072
fastaseq

go idGO:0006526; GO:0006221; GO:0006221
go termarginine biosynthesis; pyrimidine nucleotide biosynthesis; pyrimidine nucleotide biosynthesis

gene product descriptioncarbamoyl-phosphate synthase large subunit
comment gene product description
evidenceE
context
gene product descEnzyme
cell locationPeriplasmic
featuresCDS

functional category codeE; F
functional categoryAmino acid transport and metabolism ; Nucleotide transport and metabolism

reference #1Role of a carboxyl-terminal helix in the assembly, interchain interactions, and stability of aspartate transcarbamoylase.

Peterson C., Schachman H. (Proc Natl Acad Sci U S A. 1991 Jan 15; 88(2):458-62)
reference #2Domain structure of the large subunit of Escherichia coli carbamoyl phosphate synthetase. Location of the binding site for the allosteric inhibitor UMP in the COOH-terminal domain.

Rubio V., Cervera J., Lusty C., Bendala E., Britton H. (Biochemistry. 1991 Jan 29; 30(4):1068-75)
reference #3Function of serine-52 and serine-80 in the catalytic mechanism of Escherichia coli aspartate transcarbamoylase.

Xu W., Kantrowitz E. (Biochemistry. 1991 Mar 5; 30(9):2535-42)
reference #4Alterations in the energetics of the carbamoyl phosphate synthetase reaction by site-directed modification of the essential sulfhydryl group.

Mullins L., Lusty C., Raushel F. (J Biol Chem. 1991 May 5; 266(13):8236-40)
reference #5Heterotropic interactions in Escherichia coli aspartate transcarbamylase. Subunit interfaces involved in CTP inhibition and ATP activation.

Xi X., van Vliet F., Ladjimi M., de Wannemaeker B., de Staercke C., Glansdorff N., Piérard A., Cunin R., Hervé G. (J Mol Biol. 1991 Aug 5; 220(3):789-99)
reference #6Quantifying the allosteric properties of Escherichia coli carbamyl phosphate synthetase: determination of thermodynamic linked-function parameters in an ordered kinetic mechanism.

Braxton B., Mullins L., Raushel F., Reinhart G. (Biochemistry. 1992 Mar 3; 31(8):2309-16)
reference #7The binding of inosine monophosphate to Escherichia coli carbamoyl phosphate synthetase.

Thoden J., Raushel F., Wesenberg G., Holden H. (J Biol Chem. 1999 Aug 6; 274(32):22502-7)
reference #8Carbamoyl phosphate synthetase: closure of the B-domain as a result of nucleotide binding.

Thoden J., Wesenberg G., Raushel F., Holden H. (Biochemistry. 1999 Feb 23; 38(8):2347-57)
reference #9The structure of carbamoyl phosphate synthetase determined to 2.1 A resolution.

Thoden J., Raushel F., Benning M., Rayment I., Holden H. (Acta Crystallogr D Biol Crystallogr. 1999 Jan; 55(Pt 1):8-24)
reference #10The small subunit of carbamoyl phosphate synthetase: snapshots along the reaction pathway.

Thoden J., Huang X., Raushel F., Holden H. (Biochemistry. 1999 Dec 7; 38(49):16158-66)
reference #11The carB gene of Escherichia coli: a duplicated gene coding for the large subunit of carbamoyl-phosphate synthetase.

Nyunoya H., Lusty C. (Proc Natl Acad Sci U S A. 1983 Aug; 80(15):4629-33)
reference #12DNA sequence of the carA gene and the control region of carAB: tandem promoters, respectively controlled by arginine and the pyrimidines, regulate the synthesis of carbamoyl-phosphate synthetase in Escherichia coli K-12.

Piette J., Nyunoya H., Lusty C., Cunin R., Weyens G., Crabeel M., Charlier D., Glansdorff N., Piérard A. (Proc Natl Acad Sci U S A. 1984 Jul; 81(13):4134-8)
reference #13Multiple regulatory signals in the control region of the Escherichia coli carAB operon.

Bouvier J., Patte J., Stragier P. (Proc Natl Acad Sci U S A. 1984 Jul; 81(13):4139-43)
reference #14Structure of carbamoyl phosphate synthetase: a journey of 96 A from substrate to product.

Thoden J., Holden H., Wesenberg G., Raushel F., Rayment I. (Biochemistry. 1997 May 27; 36(21):6305-16)
reference #15Carbamoyl phosphate synthetase: caught in the act of glutamine hydrolysis.

Thoden J., Miran S., Phillips J., Howard A., Raushel F., Holden H. (Biochemistry. 1998 Jun 23; 37(25):8825-31)

phylogenetic profile
 
1 662 703 728 1240 838 835 580 1073 569 1066 1066 802 1 748 1 785 740 1 1065
1078 1094 1093 1094 1069 1053 1068 1066 1095 942 1101 1107 988 1018 1015 1259 1249 1231 1272 1291
1291 1269 1258 1283 77 73 74 1264 1120 1443 1441 1440 1456 1456 1494 1459 1462 1458 1445 1217
1261 1139 1182 1155 1182 1227 1221 1021 1258 1250 1372 1164 1103 1098 1091 1143 991 990 994 1022
1007 1013 938 962 66 964 1002 966 984 999 1003 999 1003 999 1 1 1 1 1 1
1 1 1003 1 992 995 993 994 993 982 982 986 988 988 993 991 998 990 978 951
1 1531 1443 1490 1635 1632 1483 1989 2093 2100 2100 66 1435 1434 1459 1516 1478 1949 1615 1590
1607 2071 2073 2071 1793 2100 2100 1837 1826 1824 1184 1464 1466 1447 1452 1974 1974 1978 665 764
764 1 50 1 52 54 54 591 1083 1001 61 1221 768 1134 1030 1 1162 76 1 1162
776 790 757 777 774 784 772 784 685 731 736 730 784 794 1 508 1112 1 434